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Biologically Multifunctional Surfaces Using Plasma Polymerization Methods
Author(s) -
Chu Liqiang,
Knoll Wolfgang,
Förch Renate
Publication year - 2006
Publication title -
plasma processes and polymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 74
eISSN - 1612-8869
pISSN - 1612-8850
DOI - 10.1002/ppap.200600010
Subject(s) - streptavidin , biofouling , polymer , plasma polymerization , polymerization , adsorption , biotin , materials science , chemistry , surface modification , chemical engineering , nanotechnology , polymer chemistry , organic chemistry , biochemistry , membrane , engineering
Summary: We report on the development of a multilayer system, which allows for specific DNA binding reactions to occur at the surface, but at the same time shows antifouling properties, thus minimizing the nonspecific adsorption of proteins (BSA). The synthesis involves the combination of plasma polymerization and streptavidin‐biotin assembly. DNA sensing is achieved using SPFS. Antifouling properties are tailored by controlling the film thickness, together with the effects of the swelling in buffer solution and by the specific surface chemical design. Preliminary hybridization experiments show promising results.The immobilization of biotin‐streptavidin within an antifouling pp‐EO2 plasma polymer.