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Acid/base Micropatterned Devices for pH‐Dependent Biosensors
Author(s) -
Valsesia Andrea,
Manso Silvan Miguel,
Ceccone Giacomo,
Gilliland Douglas,
Colpo Pascal,
Rossi François
Publication year - 2005
Publication title -
plasma processes and polymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 74
eISSN - 1612-8869
pISSN - 1612-8850
DOI - 10.1002/ppap.200400058
Subject(s) - x ray photoelectron spectroscopy , polymer , chemical force microscopy , materials science , force spectroscopy , adhesion , surface forces apparatus , chemical engineering , nanotechnology , chemistry , chemical vapor deposition , analytical chemistry (journal) , acrylic acid , deposition (geology) , atomic force microscopy , composite material , kelvin probe force microscope , chromatography , copolymer , non contact atomic force microscopy , paleontology , sediment , engineering , biology
Summary: Micropatterned surfaces of poly(acrylic acid) (PAA) and poly(allylamine) (PAL) were prepared by the combination of plasma enhanced chemical vapour deposition (PE‐CVD) and physical masking techniques. The deposition process for both polymers was optimized in order to obtain a good compromise between the stability and the specific chemical functionality of the surface. X‐ray photoelectron spectroscopy, and secondary ion mass spectrometry were used to determine the chemical characteristics of the surfaces. The use of the imaging mode provided a determination of the chemical contrasts at the micrometre scale. The evaluation of the specific interaction forces, acting between the surfaces and the COOH‐functionalized tips, as a function of the pH, was performed by chemical force microscopy. The force‐distance curves provided the basis for a semi‐quantitative calculation of the interaction forces between the tip and the surfaces. The repulsive or attractive nature of the interaction forces is due to the variation in the charge concentration on the surfaces. This allowed the determination of pH‐dependent force maps where the PAA and PAL show a contrast in the adhesion force.Optical image of the investigated area on the micropatterned PAA/PAL surface.