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Poly(2‐(dimethylamino)ethyl methacrylate)‐ b ‐poly(hydroxypropyl methacrylate) copolymers/bovine serum albumin complexes in aqueous solutions
Author(s) -
Sentoukas Theodore,
Pispas Stergios
Publication year - 2020
Publication title -
journal of polymer science
Language(s) - English
Resource type - Journals
eISSN - 2642-4169
pISSN - 2642-4150
DOI - 10.1002/pol.20200065
Subject(s) - copolymer , methacrylate , bovine serum albumin , amphiphile , polymer chemistry , aqueous solution , dynamic light scattering , chemistry , hydrodynamic radius , nanocarriers , materials science , polymer , chemical engineering , drug delivery , organic chemistry , chromatography , nanotechnology , nanoparticle , engineering
Complexation ability of poly(2‐(dimethylamino)ethyl methacrylate)‐ b ‐poly(hydroxy propyl methacrylate) (PDMAEMA‐ b ‐PHPMA) amphiphilic doubly thermo‐responsive block copolymers, and their quaternized counterparts QPDMAEMA‐ b ‐PHPMA, toward bovine serum albumin (BSA) is studied in aqueous solutions. The PDMAEMA‐ b ‐PHPMA amphiphilic block copolymers self‐assemble in nanostructured aggregates with PDMAEMA coronas having different inner structure and micro‐polarity depending on the solubilization protocol utilized when inserted in aqueous media. By incorporating different BSA concentrations, we investigate the copolymer–protein interactions by light scattering measurements in aqueous solutions in a broad temperature range, utilizing different solubilization protocols for the copolymers. Fluorescence spectroscopy and ζ‐potential measurements were also utilized to investigate the structure and properties of the copolymer/protein complexes formed in each case. Such knowledge may lead to a better understanding of the inner structure and micro polarity of the nanostructured aggregates formed by the novel (Q)PDMAEMA‐ b ‐PHPMA copolymers, along with their potential abilities in nanocarrier formation, protein complexation, stabilization, and delivery.