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Influence of solvent viscosity on the rate of hydrolysis of dipeptides by carboxypeptidase Y
Author(s) -
Kanosue Yoshifumi,
Kojima Satoshi,
Ohkata Katsuo
Publication year - 2004
Publication title -
journal of physical organic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.325
H-Index - 66
eISSN - 1099-1395
pISSN - 0894-3230
DOI - 10.1002/poc.752
Subject(s) - chemistry , hydrolysis , viscosity , solvent , carboxypeptidase a , carboxypeptidase , enzyme , intrinsic viscosity , alkyl , enzymatic hydrolysis , organic chemistry , stereochemistry , thermodynamics , polymer , physics
The influence of solvent viscosity on the rate of enzymatic hydrolysis of a series of dipeptides (Z‐Phe‐Gly, Z‐Phe‐Sar, Z‐Phe‐Ala, Z‐Phe‐NMeAla, Z‐Phe‐Aib and Z‐Phe‐Pro) by carboxypeptidase Y was investigated. The effect of solvent viscosity on the enzymatic hydrolysis revealed that whereas all k cat values decreased with viscosity, those of the N ‐alkyl peptides decreased more than those of the N ‐H peptides. The kinetic behaviour implies the involvement of conformational changes of the enzyme in terms of the ‘induced‐fit’ process. Copyright © 2004 John Wiley & Sons, Ltd.