Influence of solvent viscosity on the rate of hydrolysis of dipeptides by carboxypeptidase Y | Zendy

Kanosue Yoshifumi | Zendy; Kojima Satoshi | Zendy; Ohkata Katsuo | Zendy

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Influence of solvent viscosity on the rate of hydrolysis of dipeptides by carboxypeptidase Y

Author(s) -

Kanosue Yoshifumi,

Kojima Satoshi,

Ohkata Katsuo

Publication year - 2004

Publication title -

journal of physical organic chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.325

H-Index - 66

eISSN - 1099-1395

pISSN - 0894-3230

DOI - 10.1002/poc.752

Subject(s) - chemistry , hydrolysis , viscosity , solvent , carboxypeptidase a , carboxypeptidase , enzyme , intrinsic viscosity , alkyl , enzymatic hydrolysis , organic chemistry , stereochemistry , thermodynamics , polymer , physics

The influence of solvent viscosity on the rate of enzymatic hydrolysis of a series of dipeptides (Z‐Phe‐Gly, Z‐Phe‐Sar, Z‐Phe‐Ala, Z‐Phe‐NMeAla, Z‐Phe‐Aib and Z‐Phe‐Pro) by carboxypeptidase Y was investigated. The effect of solvent viscosity on the enzymatic hydrolysis revealed that whereas all k cat values decreased with viscosity, those of the N ‐alkyl peptides decreased more than those of the N ‐H peptides. The kinetic behaviour implies the involvement of conformational changes of the enzyme in terms of the ‘induced‐fit’ process. Copyright © 2004 John Wiley & Sons, Ltd.

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