Premium
Calorimetric study of human serum albumin in water–dioxane mixtures
Author(s) -
Borisover Mikhail D.,
Sirotkin Vladimir A.,
Solomonov Boris N.
Publication year - 1993
Publication title -
journal of physical organic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.325
H-Index - 66
eISSN - 1099-1395
pISSN - 0894-3230
DOI - 10.1002/poc.610060409
Subject(s) - chemistry , human serum albumin , aqueous solution , enthalpy , solvent , adsorption , intermolecular force , sorption , thermodynamics , water activity , monolayer , chromatography , organic chemistry , water content , molecule , physics , geotechnical engineering , engineering , biochemistry
The calorimetric isotherm of water sorption by solid human serum albumin (HSA) was measured in water–dioxane mixtures. This isotherm has been successfully described at low water contents in the solvent within the framework of the presented model. The adsorption equilibrium constant and the monolayer formation energy were estimated from this model. The interaction enthalpies of solid HSA with the solvent in water‐rich mixtures do not differ essentially from the solution enthalpy of HSA in water. This means that the solvent water content may be sufficient for the intermolecular interactions of HSA suspended in a water–organic mixture to be similar to those in aqueous protein solution.