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Protein immobilization by aminolysis of cellulose xanthate esters
Author(s) -
Humeres Eduardo,
Oliveira Célia Ma. Da Silva,
Osellame Volnei T.,
De Souza Iolanda
Publication year - 1991
Publication title -
journal of physical organic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.325
H-Index - 66
eISSN - 1099-1395
pISSN - 0894-3230
DOI - 10.1002/poc.610040908
Subject(s) - chemistry , aminolysis , cellulose , reaction rate constant , hydroxide , hydrolysis , xanthate , bovine serum albumin , inorganic chemistry , medicinal chemistry , kinetics , organic chemistry , chromatography , physics , quantum mechanics , catalysis
P ‐Nitrobenzyl cellulose xanthate (CelXNB) was obtained with a degree of substitution ( DS ) in the range 0.5–3. The first order rate constant for hydrolysis of CelXNB at 25°C, extrapolated to zero buffer concentration, is hydroxide ion catalysed, whereas the water‐catalyed path is about 2000 times faster than that observed for p ‐nitrobenzyl ethylxanthate (EXNB), probably owing to the highly ordered cybotactic region of cellulose. Aminolysis of CelXNB produces the corresponding thioncarbamate ester; for simple alkylamines, the second‐order rate constants are similar to those obtained for EXNB. The secod‐order rate constants are similar to those obtained for EXNB. The second‐order rate constants at pH 11 for immobilization of bacterial α‐amylase and bovine serum albumin were 13.4 and 112 1 mol −1 s −1 , respectively, unexpectedly high values when compared with simple alkylamines, even considering the concentration of external reactive groups of the proteins. CelXNB with low DS should release 1 mol of p ‐nitro‐α‐toluenethiol for every mole of protein that becomes immobilized. The net weight increase of the cellulose matrix allows the calculation of the absolute molecular weight of the protein. Preliminary results support this assumption.