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Mechanistic insights into the hydrolysis of organophosphorus compounds by paraoxonase‐1: exploring the limits of substrate tolerance in a promiscuous enzyme
Author(s) -
Muthukrishnan Sivaramakrishnan,
Shete Vivekanand S.,
Sanan Toby T.,
Vyas Shubham,
Oottikkal Shameema,
Porter Lauren M.,
Magliery Thomas J.,
Hadad Christopher M.
Publication year - 2012
Publication title -
journal of physical organic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.325
H-Index - 66
eISSN - 1099-1395
pISSN - 0894-3230
DOI - 10.1002/poc.3002
Subject(s) - paraoxon , chemistry , steric effects , substrate (aquarium) , organophosphate , hydrolysis , paraoxonase , stereochemistry , alkyl , active site , enzyme , aryldialkylphosphatase , organic chemistry , acetylcholinesterase , biochemistry , pesticide , oceanography , gene , genotype , agronomy , biology , geology , pon1
We designed, synthesized, and screened a library of analogs of the organophosphate pesticide metabolite paraoxon against a recombinant variant of human serum paraoxonase‐1. Alterations of both the aryloxy leaving group and the retained alkyl chains of paraoxon analogs resulted in substantial changes to binding and hydrolysis, as measured directly by spectrophotometric methods or in competition experiments with paraoxon. Increases or decreases in the steric bulk of the retained groups generally reduced the rate of hydrolysis, while modifications of the leaving group modulated both binding and turnover. Studies on the hydrolysis of phosphoryl azide analogs as well as amino‐modified paraoxon analogs, the former being developed as photoaffinity labels, found enhanced tolerance of structural modifications when compared with O ‐alkyl‐substituted molecules. Results from computational modeling predict a predominant active site binding mode for these molecules, which is consistent with several proposed catalytic mechanisms in the literature and from which a molecular‐level explanation of the experimental trends is attempted. Overall, the results of this study suggest that while paraoxonase‐1 is a promiscuous enzyme, there are substantial constraints in the active site pocket, which may relate to both the leaving group and the retained portion of paraoxon analogs. Copyright © 2012 John Wiley & Sons, Ltd.