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Carminic acid: an antioxidant to protect erythrocytes and DNA against radical‐induced oxidation
Author(s) -
Li GuoXiang,
Liu ZaiQun,
Wu Di
Publication year - 2009
Publication title -
journal of physical organic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.325
H-Index - 66
eISSN - 1099-1395
pISSN - 0894-3230
DOI - 10.1002/poc.1536
Subject(s) - chemistry , radical , abts , tbars , dpph , antioxidant , thiobarbituric acid , hydrochloride , medicinal chemistry , nuclear chemistry , hemolysis , organic chemistry , lipid peroxidation , immunology , biology
This work explored the antioxidant effect of carminic acid (CarOH) on the oxidation of DNA and erythrocytes induced by 2,2′‐azobis(2‐amidinopropane hydrochloride) (AAPH). The half concentrations (IC 50 ) of CarOH to scavenge radicals were measured by reacting with 2,2′‐azinobis(3‐ethylbenzothiazoline‐6‐sulfonate) radical cation (ABTS +• ) and 2,2′‐diphenyl‐1‐picrylhydrazyl (DPPH). The values of IC 50 were 8.0 and 26.0 µM when CarOH reacted with ABTS +• and DPPH, respectively. CarOH was able to protect DNA against AAPH‐induced oxidative damage by decreasing the formation rate of thiobarbituric acid reactive substance (TBARS). In addition, CarOH protected human erythrocytes against AAPH‐induced hemolysis concentration dependently, during which one molecule of CarOH can trap almost three radicals. Moreover, quantum calculation revealed that the hydroxyl group at 6‐position played major role in trapping radicals. Copyright © 2009 John Wiley & Sons, Ltd.