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Mass spectrometry detection of monkeypox virus: Comprehensive coverage for ranking the most responsive peptide markers
Author(s) -
Lozano Clément,
Grenga Lucia,
Gallais Fabrice,
Miotello Guylaine,
Bellanger Laurent,
Armengaud Jean
Publication year - 2023
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.202200253
Subject(s) - monkeypox , proteome , proteomics , shotgun proteomics , mass spectrometry , computational biology , shotgun , quantitative proteomics , biology , virology , bioinformatics , vaccinia , chemistry , chromatography , genetics , recombinant dna , gene
The recent and sudden outbreak of monkeypox in numerous non‐endemic countries requires expanding its surveillance immediately and understanding its origin and spread. As learned from the COVID‐19 pandemic, appropriate detection techniques are crucial to achieving such a goal. Mass spectrometry has the advantages of a rapid response, low analytical interferences, better precision, and easier multiplexing to detect various pathogens and their variants. In this proteomic dataset, we report experimental data on the proteome of the monkeypox virus (MPXV) recorded by state‐of‐the‐art shotgun proteomics, including data‐dependent and data‐independent acquisition for comprehensive coverage. We highlighted 152 viral proteins, corresponding to an overall proteome coverage of 79.5 %. Among the 1371 viral peptides detected, 35 peptides with the most intense signals in mass spectrometry were selected, representing a subset of 13 viral proteins. Their relevance as potential candidate markers for virus detection by targeted mass spectrometry is discussed. This report should assist the rapid development of mass spectrometry‐based tests to detect a pathogen of increasing concern.