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Ion mobility‐resolved phosphoproteomics with dia‐PASEF and short gradients
Author(s) -
Oliinyk Denys,
Meier Florian
Publication year - 2023
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.202200032
Subject(s) - phosphoproteomics , ion mobility spectrometry , proteomics , chemistry , computational biology , chromatography , biology , mass spectrometry , biochemistry , phosphorylation , protein phosphorylation , protein kinase a , gene
Abstract Mass spectrometry‐based phosphoproteomics has identified >150,000 post‐translational phosphorylation sites in the human proteome. To disentangle their functional relevance, complex experimental designs that require increased throughput are now coming into focus. Here, we apply dia‐PASEF on a trapped ion mobility (TIMS) mass spectrometer to analyze the phosphoproteome of a human cancer cell line in short liquid chromatography gradients. At low sample amounts equivalent to ∼20 ug protein digest per analysis, we quantified over 13,000 phosphopeptides including ∼8700 class I phosphosites in 1 h without a spectral library. Decreasing the gradient time to 15 min yielded virtually identical coverage of the phosphoproteome, and with 7 min gradients we still quantified about 80% of the class I sites with a median coefficient of variation <10% in quadruplicates. We attribute this in part to the increased peak capacity, which effectively compensates for the higher peptide density per time unit in shorter gradients. Our data show a five‐fold reduction in the number of co‐isolated peptides with TIMS. In the most extreme case, these were positional isomers of nearby phosphosites that remained unresolved with fast liquid chromatography. In summary, our study demonstrates how key features of dia‐PASEF translate to phosphoproteomics.