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Front Cover: New Approaches for Absolute Quantification of Stable‐Isotope‐Labeled Peptide Standards for Targeted Proteomics Based on a UV Active Tag
Author(s) -
Schnatbaum Karsten,
SolisMezarino Victor,
Pokrovsky Daniil,
Schäfer Frederike,
Nagl Dennis,
Hornberger Lars,
Zerweck Johannes,
Knaute Tobias,
AvramovaNehmer Julia,
Schutkowski Mike,
Hornung Veit,
Wenschuh Holger,
VölkerAlbert Moritz Carl,
Imhof Axel,
Reimer Ulf
Publication year - 2020
Publication title -
proteomics
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.202070081
Subject(s) - proteomics , quantitative proteomics , peptide , front cover , chemistry , histone , chromatography , computational biology , mass spectrometry , stable isotope ratio , biochemistry , biology , cover (algebra) , dna , mechanical engineering , engineering , gene , physics , quantum mechanics
DOI: 10.1002/pmic.2027 Targeted proteomics, a powerful strategy for simultaneous determination of human proteins, requires quantified stable isotope labeled (SIL) peptide standards to enable precise protein quantification. In article number 27 by Karsten Schnatbaum et al., a new approach for absolute quantification of SIL peptides is reported, which eliminates the need for laborious and expensive amino acid analysis based protocols. The method relies on a UV‐active quantification tag (Qtag) that is removed by tryptic digestion and was exemplarily applied for accurate quantification of the in‐vivo specificity of a histone methyltransferase.