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The Sperm Proteome of the Oyster Crassostrea hongkongensis
Author(s) -
Mu Huawei,
Ke Shengwei,
Zhang Duo,
Zhang Yanjie,
Song Xiaoyuan,
Yu Ziniu,
Zhang Yang,
Qiu JianWen
Publication year - 2020
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.202000167
Subject(s) - sperm , crassostrea , marine invertebrates , biology , proteome , mytilus , proteomics , oyster , pacific oyster , human fertilization , zoology , biochemistry , genetics , gene , ecology
Sperm proteins play vital roles in fertilization, but little is known about their identities in free‐spawning marine invertebrates. Here, 286 sperm proteins are reported from the Hong Kong oyster Crassostrea hongkongensis using label‐free and semi‐quantitative proteomics. Proteins extracted from three sperm samples are separated by SDS‐PAGE, analyzed by LC‐MS/MS, and identified using Mascot. Functional classification of the sperm proteome reveals energy metabolism (33%), signaling and binding (23%), and protein synthesis and degradation (12%) as the top functional categories. Comparison of orthologous sperm proteins between C. hongkongensis , Crassostrea gigas , Mytilus edulis , and M. galloprovincialis suggests that energy metabolism (48%) is the most conserved functional group. Sequence alignment of the C. hongkongensis bindin, an acrosomal protein that binds the sperm and the egg, with those of three other Crassostrea species, reveals several conserved motifs. The study has enriched the data of invertebrate sperm proteins and may contribute to studies of mechanisms of fertilization in free‐spawning invertebrates. The proteomic data are available in ProteomeXchange with the identifier PXD018255.