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In‐depth proteome of perilymph in guinea pig model
Author(s) -
Lee Jung Hun,
Hwang YuJung,
Li Hui,
Kim Hyeyoon,
Suh MyungWhan,
Han Dohyun,
Oh SeungHa
Publication year - 2021
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.202000138
Subject(s) - perilymph , proteome , guinea pig , cavia , inner ear , biology , sensorineural hearing loss , proteomics , computational biology , audiology , hearing loss , medicine , neuroscience , bioinformatics , biochemistry , genetics , gene
The vast majority of sensorineural hearing loss is caused by impairment of the inner ear cells. Proteomic analysis of perilymph may therefore improve our understanding of inner ear diseases and hearing loss. However, the investigation of the human perilymph proteome was limited due to technical difficulties in perilymph sampling. The guinea pig ( Cavia porcellus ) is frequently used as an experimental model in preclinical hearing research. In this study, we analyzed samples of perilymph collected from 12 guinea pigs to overcome limited experimental information regarding its proteome. We identified a total of 1413 proteins, establishing a greatly expanded proteome of the previously inferred guinea pig perilymph. This provides a comprehensive proteomic resource for the research community, which will facilitate future molecular‐phenotypic studies using the guinea pig as an experimental model of relevance to human inner ear biology.