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A Comparison of Protein and mRNA Expression during Development of the Soil Dwelling Prokaryote ( S. coelicolor )
Author(s) -
Pospíšil Jiří,
Strunin Dmytro,
Ziková Alice,
Hubálek Martin,
Vohradský Jiří
Publication year - 2020
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.202000032
Subject(s) - streptomyces coelicolor , proteome , translation (biology) , protein biosynthesis , prokaryote , messenger rna , biology , exponential growth , population , protein expression , gene expression , bacteria , gene , microbiology and biotechnology , genetics , streptomyces , mathematics , mathematical analysis , demography , sociology
In this paper, correlation analysis of protein and mRNA levels in the soil dwelling bacteria Streptomyces coelicolor ( S. coelicolor M145) is presented during development of the population as it grew in liquid medium using three biological and two technical replicates, measured during exponential growth, and its entry into the stationary phase. The proteome synthesis time series are compared with the gene expression time series measured previously under identical experimental conditions. Results reveal that about one third of protein/mRNA synthesis profiles are well correlated while another third are correlated negatively. Functional analysis of the highly correlated groups is presented. Based on numerical simulation, the negative correlation between protein and mRNA is shown to be caused by the difference between the rate of translation and protein degradation.