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Front Cover: Proteome Profile of Endogenous Retrotransposon‐Associated Complexes in Human Embryonic Stem Cells
Author(s) -
Vuong Linh M.,
Pan Songqin,
Donovan Peter J.
Publication year - 2019
Publication title -
proteomics
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201970131
Subject(s) - biology , retrotransposon , embryonic stem cell , stem cell , homeobox protein nanog , proteome , transposable element , microbiology and biotechnology , human genome , genetics , genome , gene , induced pluripotent stem cell
DOI: 10.1002/pmic.201900169 Long Interspersed Element‐1 (L1) is a major transposable element present in the human genome. L1 activity is implicated in various human diseases and cancer. Depicted here is a colony of human embryonic stem cell (ESCs) expressing the endogenous L1‐encoded ORF1 protein. ORF1p appears as distinct cytoplasmic foci (green) close to nuclei expressing the stem cell marker, OCT4 (red). Phalloidin‐Cyan (blue) stains actin. In article number 1900169, Vuong et al. identify cellular proteins associated with ORF1p in human ESCs. Understanding ORF1p function is likely critical to deciphering how the L1 element has evolved with our species and impacts our biology and physiology.