Premium
Front Cover: A Dual‐Purpose Bromocoumarin Tag Enables Deep Profiling of the Cellular Cysteinome
Author(s) -
Rabalski Adam J.,
Williams Jon D.,
McClure Ryan A.,
Vasudevan Anil,
Baranczak Aleksandra
Publication year - 2019
Publication title -
proteomics
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201970091
Subject(s) - front cover , proteome , proteomics , profiling (computer programming) , mass spectrometry , chemistry , computational biology , chromatography , biology , cover (algebra) , computer science , biochemistry , mechanical engineering , engineering , gene , operating system
DOI: 10.1002/pmic.201800433 Chemical proteomics enables comprehensive profiling of small molecules in complex proteomes. In this study, fluorescence‐triggered two‐dimensional chromatography based on the application of a bromocoumarin tag facilitates the isolation of small molecule‐conjugated peptides prior to mass spectrometry. Profiling of a cysteinome with the use of a bromocoumarin tag demonstrates that two‐dimensional fluorescence‐based chromatography separation enables deep interrogation of select portions of cellular proteome. More details can be found in article number 1800433 by Adam J. Rabalski et al.