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DNA‐Barcoded Fluorescence Microscopy for Spatial Omics
Author(s) -
Schueder Florian,
Unterauer Eduard M.,
Ganji Mahipal,
Jungmann Ralf
Publication year - 2020
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201900368
Subject(s) - proteomics , genomics , context (archaeology) , computational biology , fluorescence microscope , data science , toolbox , biology , computer science , genome , fluorescence , genetics , physics , paleontology , quantum mechanics , gene , programming language
Innovation in genomics, transcriptomics, and proteomics research has created a plethora of state‐of‐the‐art techniques such as nucleic acid sequencing and mass‐spectrometry‐based proteomics with paramount impact in the life sciences. While current approaches yield quantitative abundance analysis of biomolecules on an almost routine basis, coupling this high content to spatial information in a single cell and tissue context is challenging. Here, current implementations of spatial omics are discussed and recent developments in the field of DNA‐barcoded fluorescence microscopy are reviewed. Light is shed on the potential of DNA‐based imaging techniques to provide a comprehensive toolbox for spatial genomics and transcriptomics and discuss current challenges, which need to be overcome on the way to spatial proteomics using high‐resolution fluorescence microscopy.