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Pan Proteome of Xanthomonas campestris pv. campestris Isolates Contrasting in Virulence
Author(s) -
Távora Fabiano T. P. K.,
Santos Cristiane,
Maximiano Mariana R.,
Murad André M.,
OliveiraNeto Osmundo Brilhante,
Megias Esaú,
Reis Junior Fábio B.,
Franco Octávio L.,
Mehta Angela
Publication year - 2019
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201900082
Subject(s) - xanthomonas campestris , virulence , xanthomonas campestris pv. campestris , biology , proteome , microbiology and biotechnology , xanthomonas , botany , pathogen , bacteria , genetics , gene
Fully sequenced genomes of X anthomonas campestris pv. campestris ( Xcc ) strains are reported. However, intra‐pathovar differences are still intriguing and far from clear. In this work, the contrasting virulence between two isolates of Xcc ‐ Xcc51 (more virulent) and XccY21 (less virulent) is evaluated by determining their pan proteome profiles. The bacteria are grown in NYG and XVM1 (optimal for induction of hrp regulon) broths and collected at the max‐exponential growth phase. Shotgun proteomics reveals a total of 329 proteins when Xcc isolates are grown in XVM1. A comparison of both profiles reveals 47 proteins with significant abundance fluctuations, out of which, 39 show an increased abundance in Xcc 51 and are mainly involved in virulence/adaptation mechanisms, genetic information processing, and membrane receptor/iron transport systems, such as BfeA, BtuB, Cap, Clp, Dcp, FyuA, GroEs, HpaG, Tig, and OmpP6. Several differential proteins are further analyzed by qRT‐PCR, which reveals a similar expression pattern to the protein abundance. The data shed light on the complex Xcc pathogenicity mechanisms and point out a set of proteins related to the higher virulence of Xcc 51. This information is essential for the development of more efficient strategies aiming at the control of black rot disease.