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Protein Paucimannosylation Is an Enriched N ‐Glycosylation Signature of Human Cancers
Author(s) -
Chatterjee Sayantani,
Lee Ling Y.,
Kawahara Rebeca,
Abrahams Jodie L.,
Adamczyk Barbara,
Anugraham Merrina,
Ashwood Christopher,
SumerBayraktar Zeynep,
Briggs Matthew T.,
Chik Jenny H. L.,
EverestDass Arun,
Förster Sarah,
Hinneburg Hannes,
Leite Katia R. M.,
Loke Ian,
Möginger Uwe,
Moh Edward S. X.,
Nakano Miyako,
Recuero Saulo,
Sethi Manveen K.,
Srougi Miguel,
Stavenhagen Kathrin,
Venkatakrishnan Vignesh,
WongtrakulKish Katherine,
Diestel Simone,
Hoffmann Peter,
Karlsson Niclas G.,
Kolarich Daniel,
Molloy Mark P.,
Muders Michael H.,
Oehler Martin K.,
Packer Nicolle H.,
Palmisano Giuseppe,
ThaysenAndersen Morten
Publication year - 2019
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201900010
Subject(s) - glycomics , carcinogenesis , cancer , biology , glycome , cancer research , glycosylation , ovarian cancer , proteomics , cancer cell , metastasis , prostate cancer , glycoprotein , glycan , microbiology and biotechnology , genetics , gene
While aberrant protein glycosylation is a recognized characteristic of human cancers, advances in glycoanalytics continue to discover new associations between glycoproteins and tumorigenesis. This glycomics‐centric study investigates a possible link between protein paucimannosylation, an under‐studied class of human N ‐glycosylation [Man 1‐3 GlcNAc 2 Fuc 0‐1 ], and cancer. The paucimannosidic glycans (PMGs) of 34 cancer cell lines and 133 tissue samples spanning 11 cancer types and matching non‐cancerous specimens are profiled from 467 published and unpublished PGC‐LC‐MS/MS N ‐glycome datasets collected over a decade. PMGs, particularly Man 2‐3 GlcNAc 2 Fuc 1 , are prominent features of 29 cancer cell lines, but the PMG level varies dramatically across and within the cancer types (1.0–50.2%). Analyses of paired (tumor/non‐tumor) and stage‐stratified tissues demonstrate that PMGs are significantly enriched in tumor tissues from several cancer types including liver cancer ( p = 0.0033) and colorectal cancer ( p = 0.0017) and is elevated as a result of prostate cancer and chronic lymphocytic leukaemia progression ( p < 0.05). Surface expression of paucimannosidic epitopes is demonstrated on human glioblastoma cells using immunofluorescence while biosynthetic involvement of N ‐acetyl‐β‐hexosaminidase is indicated by quantitative proteomics. This intriguing association between protein paucimannosylation and human cancers warrants further exploration to detail the biosynthesis, cellular location(s), protein carriers, and functions of paucimannosylation in tumorigenesis and metastasis.