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Identification of Saliva Proteins of the Spider Mite Tetranychus evansi by Transcriptome and LC–MS/MS Analyses
Author(s) -
Huang HaiJian,
Cui JiaRong,
Chen Lei,
Zhu YuXi,
Hong XiaoYue
Publication year - 2019
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201800302
Subject(s) - spider mite , biology , saliva , transcriptome , tetranychus , tetranychus urticae , mite , trypanosoma evansi , botany , genetics , gene , biochemistry , virology , gene expression , trypanosomiasis
The spider mite Tetranychus evansi has a remarkable ability to suppress and manipulate plant defenses, which makes it an ideal model to investigate plant–herbivores interactions. In this study, a de novo assembly of the transcriptome of T. evansi is performed and the proteins in its secreted saliva by LC–MS/MS are characterized. A total of 29 365 unigenes are assembled and 136 saliva proteins are identified. Comparative analysis of the saliva proteins in T. evansi , T. truncatus , and T. urticae shows that 64 protein groups are shared by at least two Tetranychus species, and 52 protein groups are specifically identified in T. evansi . In addition, some saliva proteins are common in arthropod species, while others are species‐specific. These results will help to elucidate the molecular mechanisms by which T. evansi modulates plant defenses.