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The Utility of Nanopore Technology for Protein and Peptide Sensing
Author(s) -
Robertson Joseph W. F.,
Reiner Joseph E.
Publication year - 2018
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201800026
Subject(s) - nanopore , peptide , analyte , nanotechnology , protein detection , characterization (materials science) , chemistry , biophysics , materials science , biochemistry , biology , chromatography
Resistive pulse nanopore sensing enables label‐free single‐molecule analysis of a wide range of analytes. An increasing number of studies have demonstrated the feasibility and usefulness of nanopore sensing for protein and peptide characterization. Nanopores offer the potential to study a variety of protein‐related phenomena that includes unfolding kinetics, differences in unfolding pathways, protein structure stability, and free‐energy profiles of DNA–protein and RNA–protein binding. In addition to providing a tool for fundamental protein characterization, nanopores have also been used as highly selective protein detectors in various solution mixtures and conditions. This review highlights these and other developments in the area of nanopore‐based protein and peptide detection.