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An Insight into Powdery Mildew–Infected, Susceptible, Resistant, and Immune Sunflower Genotypes
Author(s) -
Kallamadi Prathap Reddy,
Dandu Kamakshi,
Kirti Pulugurtha Bharadwaja,
Rao Chintalagiri Mohan,
Thakur Suman S,
Mulpuri Sujatha
Publication year - 2018
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201700418
Subject(s) - biology , powdery mildew , plant disease resistance , proteome , immune system , glutamine synthetase , formate dehydrogenase , genotype , microbiology and biotechnology , botany , genetics , biochemistry , gene , enzyme , amino acid , glutamine , cofactor
Abstract Powdery mildew (PM, caused by Golovinomyces orontii ) is one of the major diseases on sunflower that causes severe yield losses in the tropics. Sources of resistance to PM are reported in an exotic accession and some wild Helianthus species. The present study aims at quantitative proteomic analysis of susceptible, resistant, and immune genotypes of sunflower in response to PM infection at 3, 7, 10 days post infection. The majority of differentially expressed proteins in the resistant genotype belonged to oxidative stress (catalase, ATP‐sulfurylase, and formate dehydrogenase), defense (HSP‐70, heat shock transcription factors), and photosynthesis (LHCB3). In case of immune genotype, 50% of proteins are related to photosynthesis, which play a key role in plant immunity, whereas a few similar proteins are also expressed in the susceptible genotype, but in their reduced abundance besides being inadequate in timing of expression probably leading to its susceptibility to PM. KEGG enrichment analysis shows that carbon metabolism (6‐phosphogluconate dehydrogenase, pyruvate dehydrogenase, glutamine synthetase), photosynthesis, and plant–pathogen protein pathways are key pathways governing the resistance. The transcriptional expression of eight of nine differentially expressed proteins are in agreement with the expression of proteins at the corresponding time. The present study provides information on the key proteins that are upregulated in resistant and immune genotypes which restrict the disease progression and constitutes the first quantitative proteomic data of sunflower‐PM infection process.