Premium
Specific Isolation of Glycoproteins with Mesoporous Zirconia‐Polyoxometalate Hybrid
Author(s) -
Zhang Dandan,
Wang Mengmeng,
Guo Zhiyong,
Guo Pengfei,
Chen Xuwei,
Wang Jianhua
Publication year - 2018
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201700381
Subject(s) - polyoxometalate , thermogravimetric analysis , chemistry , crystallography , materials science , organic chemistry , catalysis
A novel mesoporous zirconia‐polyoxometalate ZrO 2 ‐P 8 W 48 hybrid was prepared using a surfactant‐assisted solvent evaporation technique. The acid–base reaction between the Zr‐OH groups of zirconium oxides and P 8 W 48 was followed by self‐assembly with an amphiphilic triblock copolymer as template to obtain a polyoxometalate‐based hybrid. The ZrO 2 ‐P 8 W 48 hybrid was characterized by Fourier‐transform infrared spectroscopy (FT‐IR), thermal gravimetric assay (TGA), scanning electron microscopy (SEM), high‐resolution transmission electron microscopy (HR‐TEM), energy‐dispersive X‐ray spectroscopy (EDXS), X‐ray diffraction (XRD), and nitrogen sorption/desorption. Owing to the multiple hydrogen‐bonding interactions between the P 8 W 48 moiety and the hydroxyl groups of glycoproteins, the ZrO 2 ‐P 8 W 48 hybrid exhibited highly selective isolation of glycoproteins from complex matrices that included various non‐glycoproteins. The retained glycoproteins could be readily recovered using a 0.01 mol L −1 cetane trimethyl ammonium bromide (CTAB) solution as stripping reagent, with recovery rates of 92, 100, 100, 100, and 74% for the five target glycoproteins, Ovalbumin (Ova), conalbumin (ConA), immunoglobulin G from human serum (IgG), γ‐globulin from bovine milk (γ‐Glo), and horseradish peroxidase (HRP), respectively. The ZrO 2 ‐P 8 W 48 hybrid was successfully applied to the isolation of glycoproteins from egg white and human serum samples, as confirmed by SDS‐PAGE and LC‐MS/MS assays.