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Proteogenomic Insights into the Intestinal Parasite Blastocystis sp. Subtype 4 Isolate WR1
Author(s) -
Armengaud Jean,
Pible Olivier,
Gaillard JeanCharles,
Cian Amandine,
Gantois Nausicaa,
Tan Kevin S. W.,
Chabé Magali,
Viscogliosi Eric
Publication year - 2017
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201700211
Subject(s) - proteogenomics , biology , biogenesis , proteomics , genome , proteome , genetics , starfish , computational biology , apobec , interactome , gene , genomics , ecology
Abstract Blastocystis sp. is known for years as a highly prevalent anaerobic eukaryotic parasite of humans and animals. Several monophyletic clades have been delineated based on molecular data, and the occurrence of each subtype in humans and/or animal hosts has been documented. The genome of several representatives has been sequenced revealing specific traits such as an intriguing 3'‐end processing of primary transcripts. Here, a first high‐throughput proteomics dataset acquired on this difficult‐to‐cultivate parasite is presented for the zoonotic subtype T4 isolate WR1. Amongst the 2766 detected proteins, we highlighted the role of a small ADP ribosylation factor GTP‐binding protein involved in intracellular traffic as major regulator of vesicle biogenesis and a voltage‐dependent anion‐selective channel protein because both were unexpectedly highly abundant. We show how these data may be used for gaining proteogenomics insights into Blastocystis sp. specific molecular mechanisms. We evidenced for the first time by proteogenomics a functional termination codon derived from transcript polyadenylation for seven different key cellular components.