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Front cover: Sweet characterisation of prostate specific antigen using electrochemical lectin‐based immunosensor assay and MALDI TOF/TOF analysis: Focus on sialic acid
Author(s) -
Pihikova Dominika,
Pakanova Zuzana,
Nemcovic Marek,
Barath Peter,
Belicky Stefan,
Bertok Tomas,
Kasak Peter,
Mucha Jan,
Tkac Jan
Publication year - 2016
Publication title -
proteomics
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201670310
Subject(s) - sialic acid , lectin , glycan , chemistry , prostate cancer , biochemistry , chromatography , front cover , prostate specific antigen , mass spectrometry , computational biology , combinatorial chemistry , glycoprotein , cancer , biology , medicine , cover (algebra) , mechanical engineering , engineering
DOI: 10.1002/pmic.201500463 Mass spectrometry analysis of different linkages sialic acid is attached to the glycan backbone is a daunting task requiring derivatisation steps. In this work we applied lectins specifically recognizing either α‐(2,3)‐ or α‐(2,6)‐linked terminal sialic acid for specific glycoprofiling of a prostate specific antigen (a prostate cancer biomarker) using immunosensors based on electrochemical detection. Both methods were directly compared with a good agreement suggesting that electrochemical lectin‐based immunosensor has potential to be applied for prostate cancer diagnosis. For more details, see the article by Dominika Pihikova et al. on page 3085.