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Inside Front Cover: Multi‐mode acquisition (MMA): An MS/MS acquisition strategy for maximizing selectivity, specificity and sensitivity of DIA product ion spectra
Author(s) -
Williams Brad J.,
Ciavarini Steve J.,
Devlin Curt,
Cohn Steven M.,
Xie Rong,
Vissers Johannes P.C.,
Martin LeRoy B.,
Caswell Allen,
Langridge James I.,
Geromanos Scott J.
Publication year - 2016
Publication title -
proteomics
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201670144
Subject(s) - chemistry , front cover , fragmentation (computing) , ion , mass spectrum , mass spectrometry , analytical chemistry (journal) , selectivity , spectral line , chromatography , product (mathematics) , sensitivity (control systems) , cover (algebra) , physics , computer science , organic chemistry , mathematics , geometry , catalysis , engineering , operating system , astronomy , mechanical engineering , electronic engineering
DOI: 10.1002/pmic.201500492 There is a fixed amount of m/z sufficient space in an MS/MS spectrum. The number of precursors that can accurately identified is a function of the pre‐fragmentation resolving powers ( m/z , chromatographic and drift). Precursor ion flux is not uniform across time, drift or m/z . This variability necessitates the width of the mass isolation window (MIW) to be dynamic. The described MMA workflow uses models and prior knowledge to dynamically set the MIW over the gradient elution. For more details, see the article by Brad J. Williams et al. on pages 2284–2301.