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Cell wall proteome analysis of Arabidopsis thaliana mature stems
Author(s) -
Duruflé Harold,
Clemente Hélène San,
Balliau Thierry,
Zivy Michel,
Dunand Christophe,
Jamet Elisabeth
Publication year - 2017
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201600449
Subject(s) - brachypodium distachyon , proteome , cell wall , proteases , arabidopsis thaliana , biology , microbiology and biotechnology , medicago sativa , linum , arabidopsis , botany , biochemistry , genome , mutant , enzyme , gene
Plant stems carry flowers necessary for species propagation and need to be adapted to mechanical disturbance and environmental factors. The stem cell walls are different from other organs and can modify their rigidity or viscoelastic properties for the integrity and the robustness required to withstand mechanical impacts and environmental stresses. Plant cell wall is composed of complex polysaccharide networks also containing cell wall proteins (CWPs) crucial to perceive and limit the environmental effects. The CWPs are fundamental players in cell wall remodeling processes, and today, only 86 have been identified from the mature stems of the model plant Arabidopsis thaliana . With a destructive method, this study has enlarged its coverage to 302 CWPs. This new proteome is mainly composed of 27.5% proteins acting on polysaccharides, 16% proteases, 11.6% oxido‐reductases, 11% possibly related to lipid metabolism and 11% of proteins with interacting domains with proteins or polysaccharides. Compared to stem cell wall proteomes already available ( Brachypodium distachyon , Sacharum officinarum, Linum usitatissimum, Medicago sativa ), that of A. thaliana stems has a higher proportion of proteins acting on polysaccharides and of proteases, but a lower proportion of oxido‐reductases.