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Proteomic analysis of human follicular fluid in poor ovarian responders during in vitro fertilization
Author(s) -
Oh Jae Won,
Kim Seul Ki,
Cho KyungCho,
Kim MinSik,
Suh Chang Suk,
Lee Jung Ryeol,
Kim Kwang Pyo
Publication year - 2017
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201600333
Subject(s) - in vitro fertilisation , follicular fluid , proteomics , biology , andrology , follicular phase , embryo transfer , human fertilization , in vitro , embryo , endocrinology , microbiology and biotechnology , medicine , oocyte , biochemistry , anatomy , gene
Poor ovarian response (POR) in controlled ovarian stimulation is often observed during in vitro fertilization and embryo transfer cycles and it is a major problem. A POR has been found to be related to several factors, including advanced age, high body mass index, and history of ovarian or pelvic surgery. However, it is difficult to predict POR, as there are no specific biomarkers known. In this study, we used quantitative proteomic analyses to investigate potential biomarkers that can predict poor response during in vitro fertilization based on follicular fluid samples. A total of 1079 proteins were identified using a high‐resolution orbitrap mass spectrometer coupled online to a nanoflow‐LC system. It is notable that 65 upregulated and 66 downregulated proteins were found to be functionally enriched in poor responders. We also validated these differentially expressed proteins using a triple‐quadrupole mass spectrometer for quantification of targeted proteins. Of the differentially expressed proteins, three proteins (pregnancy zone protein, renin, and sushi repeat‐containing protein SRPX) were regarded as statistically significant ( p < 0.05).