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Protein microarray applications: Autoantibody detection and posttranslational modification
Author(s) -
Atak Apurva,
Mukherjee Shuvolina,
Jain Rekha,
Gupta Shabarni,
Singh Vedita Anand,
Gahoi Nikita,
K. P. Manubhai,
Srivastava Sanjeeva
Publication year - 2016
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201600104
Subject(s) - protein microarray , antibody microarray , proteomics , dna microarray , computational biology , protein array analysis , microarray , biology , antibody , genetics , gene , gene expression
The discovery of DNA microarrays was a major milestone in genomics; however, it could not adequately predict the structure or dynamics of underlying protein entities, which are the ultimate effector molecules in a cell. Protein microarrays allow simultaneous study of thousands of proteins/peptides, and various advancements in array technologies have made this platform suitable for several diagnostic and functional studies. Antibody arrays enable researchers to quantify the abundance of target proteins in biological fluids and assess PTMs by using the antibodies. Protein microarrays have been used to assess protein–protein interactions, protein–ligand interactions, and autoantibody profiling in various disease conditions. Here, we summarize different microarray platforms with focus on its biological and clinical applications in autoantibody profiling and PTM studies. We also enumerate the potential of tissue microarrays to validate findings from protein arrays as well as other approaches, highlighting their significance in proteomics.