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Human aqueous humor proteome in cataract, glaucoma, and pseudoexfoliation syndrome
Author(s) -
Kliuchnikova Anna A.,
Samokhidezhda I.,
Ilina Irina Y.,
Karpov Dmitry S.,
Pyatnitskiy Mikhail A.,
Kuznetsova Ksenia G.,
Toropygin Ilya Y.,
Kochergin Sergey A.,
Alekseev Igor B.,
Zgoda Victor G.,
Archakov Alexander I.,
Moshkovskii Sergei A.
Publication year - 2016
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201500423
Subject(s) - pseudoexfoliation syndrome , proteome , aqueous humor , glaucoma , proteomics , ophthalmology , pseudoexfoliation , medicine , chemistry , biochemistry , gene
Twenty‐nine human aqueous humor samples from patients with eye diseases such as cataract and glaucoma with and without pseudoexfoliation syndrome were characterized by LC–high resolution MS analysis. In total, 269 protein groups were identified with 1% false discovery rate including 32 groups that were not reported previously for this biological fluid. Since the samples were analyzed individually, but not pooled, 36 proteins were identified in all samples, comprising the constitutive proteome of the fluid. The most dominant molecular function of aqueous humor proteins as determined by GO analysis is endopeptidase inhibitor activity. Label‐free protein quantification showed no significant difference between glaucoma and cataract aqueous humor proteomes. At the same time, we found decrease in the level of apolipoprotein D as a marker of the pseudoexfoliation syndrome. The data are available from ProteomeXchange repository (PXD002623).