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Effects of stem cell therapy on protein profile of parkinsonian rats using an 18 O‐labeling quantitative proteomic approach
Author(s) -
Liu Yahui,
Liu Kefu,
Qin Wei,
Liu Chenghao,
Zheng Xiaowei,
Deng Yulin,
Qing Hong
Publication year - 2016
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201500421
Subject(s) - proteome , proteomics , transplantation , western blot , neural stem cell , quantitative proteomics , parkinson's disease , cell , medicine , disease , stem cell , bioinformatics , pharmacology , biology , biochemistry , microbiology and biotechnology , gene
The application of neural stem cell (NSC) research to neurodegenerative diseases has led to promising clinical trials. Currently, NSC therapy is most promising for Parkinson's disease (PD). We conducted behavioral tests and immunoassays for the profiling of a PD model in rats to assess the therapeutic effects of NSC treatments. Further, using a multiple sample comparison workflow, combined with 18 O‐labeled proteome mixtures, we compared the differentially expressed proteins from control, PD, and NSC‐treated PD rats. The results were analyzed bioinformatically and verified by Western blot. Based on our initial findings, we believe that the proteomic approach is a valuable tool in evaluating the therapeutic effects of NSC transplantation on neurodegenerative disorders.