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Capillary zone electrophoresis for bottom‐up analysis of complex proteomes
Author(s) -
Sun Liangliang,
Zhu Guijie,
Yan Xiaojing,
Zhang Zhenbin,
Wojcik Roza,
Champion Matthew M.,
Dovichi Norman J.
Publication year - 2016
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201500339
Subject(s) - capillary electrophoresis , proteome , proteomics , chromatography , computational biology , chemistry , biology , biochemistry , gene
Capillary zone electrophoresis (CZE) is emerging as a useful tool in proteomic analysis. Interest arises from dramatic improvements in performance that result from improvements in the background electrolyte used for the separation, the incorporation of advanced sample injection methods, the development of robust and sensitive electrospray interfaces, and the coupling with Orbitrap mass spectrometers with high resolution and sensitivity. The combination of these technologies produces performance that is rapidly approaching the performance of UPLC‐based methods for microgram samples and exceeds the performance of UPLC‐based methods for mid‐ to low nanogram samples. These systems now produce over 10 000 peptide IDs in a single 100‐min analysis of the HeLa proteome.