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Design and synthesis of magnetic binary metal oxides nanocomposites through dopamine chemistry for highly selective enrichment of phosphopeptides
Author(s) -
Wang Mengyi,
Sun Xueni,
Li Yan,
Deng Chunhui
Publication year - 2016
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201500277
Subject(s) - nanocomposite , superparamagnetism , biocompatibility , metal , selectivity , materials science , graphene , chemical engineering , chemistry , nanotechnology , nuclear chemistry , magnetization , organic chemistry , catalysis , metallurgy , engineering , physics , quantum mechanics , magnetic field
In this work, for the first time, magnetic binary metal oxides nanocomposites which integrated Zr and Ti into one entity on an atomic scale on polydopamine coated magnetic graphene (magG/PD/(Zr‐Ti)O 4 ) was designed and synthesized, and applied to the enrichment of phosphopeptides. The newly prepared magG/PD/(Zr‐Ti)O 4 composites gathered the advantages of large surface area, superparamagnetism, biocompatibility and the enhanced affinity properties to phosphopeptides. MagG/PD/ZrO 2 , magG/PD/TiO 2 , as well as the simple physical mixture of them were introduced to compare with magG/PD/(Zr‐Ti)O 4 composites. High sensitivity (1 pg/μL or 4.0 × 10 –11 M) and selectivity (weight ratio of β‐casein and BSA reached up to 1:8000) toward phosphopeptides were also presented for magG/PD/(Zr‐Ti)O 4 composites. Additionally, mouse brain tissue was chose as the real samples to further investigate the phosphopeptides enrichment ability of this new material.