Premium
Proteomic analysis of a compatible interaction between sugarcane and Sporisorium scitamineum
Author(s) -
Barnabas Leonard,
Ashwin N. M. R.,
Kaverinathan K.,
Trentin Anna Rita,
Pivato Micaela,
Sundar A. Ramesh,
Malathi P.,
Viswanathan R.,
Rosana O. B.,
Neethukrishna K.,
Carletti Paolo,
Arrigoni Giorgio,
Masi Antonio,
Agrawal Ganesh Kumar,
Rakwal Randeep
Publication year - 2016
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201500245
Subject(s) - biology , meristem , proteome , proteomics , smut , botany , genetics , gene
Smut caused by Sporisorium scitamineum is one of the important diseases of sugarcane with global significance. Despite the intriguing nature of sugarcane, S. scitamineum interaction, several pertinent aspects remain unexplored. This study investigates the proteome level alterations occurring in the meristem of a S. scitamineum infected susceptible sugarcane cultivar at whip emergence stage. Differentially abundant proteins were identified by 2DE coupled with MALDI‐TOF/TOF‐MS. Comprehensively, 53 sugarcane proteins identified were related to defence, stress, metabolism, protein folding, energy, and cell division; in addition, a putative effector of S. scitamineum , chorismate mutase, was identified. Transcript expression vis‐à‐vis the activity of phenylalanine ammonia lyase was relatively higher in the infected meristem. Abundance of seven candidate proteins in 2D gel profiles was in correlation with its corresponding transcript expression levels as validated by qRT‐PCR. Furthermore, this study has opened up new perspectives on the interaction between sugarcane and S. scitamineum .