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Proteomic analyses provide new insights into the responses of Pinus massoniana seedlings to phosphorus deficiency
Author(s) -
Fan Fuhua,
Ding Guijie,
Wen Xiaopeng
Publication year - 2016
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201500140
Subject(s) - pinus massoniana , phosphorus deficiency , phosphorus , biology , phosphate , metabolism , signal transduction , metabolic pathway , microbiology and biotechnology , proteome , protein biosynthesis , photosynthesis , protein metabolism , biochemistry , botany , chemistry , nutrient , ecology , organic chemistry
Phosphorus is an essential macronutrient for plant growth and development. Plants can respond defensively to phosphorus deficiency by modifying their morphology and metabolic pathways via the differential expression of low phosphate responsive genes. To better understand the mechanisms by which the Masson pine ( Pinus massoniana ) adapts to phosphorus deficiency, we conducted comparative proteomic analysis using an elite line exhibiting high tolerance to phosphorus deficiency. The selected seedlings were treated with 0.5 mM KH 2 PO 4 (control), 0.01 mM KH 2 PO 4 (P1), or 0.06 mM KH 2 PO 4 (P2) for 48 days. Total protein samples were separated via 2DE. A total of 98 differentially expressed proteins, which displayed at least 1.7‐fold change expression compared to the control levels ( p ≤ 0.05), were identified by MALDI‐TOF/TOF MS. These phosphate starvation responsive proteins were implicated in photosynthesis, defense, cellular organization, biosynthesis, energy metabolism, secondary metabolism, signal transduction etc. Therefore, these proteins might play important roles in facilitating internal phosphorus homeostasis. Additionally, the obtained data may be useful for the further characterization of gene function and may provide a foundation for a more comprehensive understanding of the adaptations of the Masson pine to phosphorus‐deficient conditions.

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