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Enhancing bottom‐up and top‐down proteomic measurements with ion mobility separations
Author(s) -
Baker Erin Shammel,
BurnumJohnson Kristin E.,
Ibrahim Yehia M.,
Orton Daniel J.,
Monroe Matthew E.,
Kelly Ryan T.,
Moore Ronald J.,
Zhang Xing,
Théberge Roger,
Costello Catherine E.,
Smith Richard D.
Publication year - 2015
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201500048
Subject(s) - ion mobility spectrometry , proteomics , ion , chemistry , computational biology , chromatography , biology , mass spectrometry , biochemistry , gene , organic chemistry
Proteomic measurements with greater throughput, sensitivity, and structural information are essential for improving both in‐depth characterization of complex mixtures and targeted studies. While LC separation coupled with MS (LC–MS) measurements have provided information on thousands of proteins in different sample types, the introduction of a separation stage that provides further component resolution and rapid structural information has many benefits in proteomic analyses. Technical advances in ion transmission and data acquisition have made ion mobility separations an opportune technology to be easily and effectively incorporated into LC–MS proteomic measurements for enhancing their information content. Herein, we report on applications illustrating increased sensitivity, throughput, and structural information by utilizing IMS–MS and LC–IMS–MS measurements for both bottom‐up and top‐down proteomics measurements.