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Proteomic analysis of extracellular vesicles derived from Mycobacterium tuberculosis
Author(s) -
Lee Jaewook,
Kim SiHyun,
Choi DongSic,
Lee Jong Seok,
Kim DaeKyum,
Go Gyeongyun,
Park SeonMin,
Kim Si Hyun,
Shin Jeong Hwan,
Chang Chulhun L.,
Gho Yong Song
Publication year - 2015
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201500037
Subject(s) - extracellular vesicles , mycobacterium tuberculosis , proteomics , microbiology and biotechnology , extracellular , chemistry , extracellular vesicle , tuberculosis , biology , computational biology , microvesicles , biochemistry , medicine , pathology , microrna , gene
The release of extracellular vesicles, also known as outer membrane vesicles, membrane vesicles, exosomes, and microvesicles, is an evolutionarily conserved phenomenon from bacteria to eukaryotes. It has been reported that Mycobacterium tuberculosis releases extracellular vesicles harboring immunologically active molecules, and these extracellular vesicles have been suggested to be applicable in vaccine development and biomarker discovery. However, the comprehensive proteomic analysis has not been performed for M. tuberculosis extracellular vesicles. In this study, we identified a total of 287 vesicular proteins by four LC-MS/MS analyses with high confidence. In addition, we identified several vesicular proteins associated with the virulence of M. tuberculosis. This comprehensive proteome profile will help elucidate the pathogenic mechanism of M. tuberculosis. The data have been deposited to the ProteomeXchange with identifier PXD001160 (http://proteomecentral.proteomexchange.org/dataset/PXD001160).