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Proteomic analysis of the herpes simplex virus 1 virion protein 16 transactivator protein in infected cells
Author(s) -
Oh Hyung Suk,
Knipe David M.
Publication year - 2015
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201500020
Subject(s) - transactivation , viral tegument , herpes simplex virus , biology , proteome , viral protein , microbiology and biotechnology , virology , virus , gene , gene expression , genetics
The herpes simplex virus 1 virion protein 16 (VP16) tegument protein forms a transactivation complex with the cellular proteins host cell factor 1 (HCF‐1) and octamer‐binding transcription factor 1 (Oct‐1) upon entry into the host cell. VP16 has also been shown to interact with a number of virion tegument proteins and viral glycoprotein H to promote viral assembly, but no comprehensive study of the VP16 proteome has been performed at early times postinfection. We therefore performed a proteomic analysis of VP16‐interacting proteins at 3 h postinfection. We confirmed the interaction of VP16 with HCF‐1 and a large number of cellular Mediator complex proteins, but most surprisingly, we found that the major viral protein associating with VP16 is the infected cell protein 4 (ICP4) immediate‐early (IE) transactivator protein. These results raise the potential for a new function for VP16 in associating with the IE ICP4 and playing a role in transactivation of early and late gene expression, in addition to its well‐documented function in transactivation of IE gene expression.