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A proteomic approach to discover and compare interacting partners of papillomavirus E2 proteins from diverse phylogenetic groups
Author(s) -
Jang Moon Kyoo,
Anderson D. Eric,
Doorslaer Koenraad,
McBride Alison A.
Publication year - 2015
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201400613
Subject(s) - biology , genome , phylogenetic tree , transcription (linguistics) , papillomaviridae , genetics , proteomics , chromatin , gene , computational biology , virology , cervical cancer , linguistics , philosophy , cancer
Papillomaviruses are a very successful group of viruses that replicate persistently in localized regions of the stratified epithelium of their specific host. Infection results in pathologies ranging from asymptomatic infection, benign warts, to malignant carcinomas. Despite this diversity, papillomavirus genomes are small (7–8 kbp) and contain at most eight genes. To sustain the complex papillomaviral life cycle, each viral protein has multiple functions and interacts with and manipulates a plethora of cellular proteins. In this study, we use tandem affinity purification and MS to identify host factors that interact with 11 different papillomavirus E2 proteins from diverse phylogenetic groups. The E2 proteins function in viral transcription and replication and correspondingly interact with host proteins involved in transcription, chromatin remodeling and modification, replication, and RNA processing.