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Proteomics equipped with multiplexing toward ultra high throughput
Author(s) -
Kim MinSik
Publication year - 2015
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201400593
Subject(s) - proteomics , proteome , computational biology , throughput , quantitative proteomics , biology , phosphorylation , computer science , bioinformatics , microbiology and biotechnology , biochemistry , telecommunications , gene , wireless
MS‐based quantitative proteomics is a powerful technology to study virtually almost all biological and clinical samples. Although it has been known to be a high‐throughput method, an MS analysis of a higher number of samples remains to be challenging practically and economically. In this issue, the use of multiplexing strategy for quantitative analysis of proteomes and phosphoproteomes has been demonstrated by Paulo et al. ( Proteomics 2015, 15 , 462–473) to better understand in vivo effects of two small molecule inhibitors on a mouse model. Within the short period of drug treatment, it has been found that the protein alteration is minimal in three tissues tested, whereas the phosphorylation level was widely altered.