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Large‐scale identification of secretome glycoproteins recognized by Wisteria floribunda agglutinin: A glycoproteomic approach to biomarker discovery
Author(s) -
Sugahara Daisuke,
Tomioka Azusa,
Sato Takashi,
Narimatsu Hisashi,
Kaji Hiroyuki
Publication year - 2015
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201400443
Subject(s) - glycan , glycoprotein , glycoproteomics , biology , biomarker , computational biology , identification (biology) , transmembrane protein , biomarker discovery , glycosylation , proteomics , biochemistry , gene , receptor , botany
One of the promising approaches to the development of cancer diagnostic systems is quantification of a specific protein carrying cancerous glycans. Potential utility of Wisteria floribunda agglutinin (WFA) for such assays has been suggested for several cancer types. To develop such diagnostic systems, identification of WFA‐recognized glycoproteins is essential. Here, we successfully identified 504 WFA‐recognized glycoproteins from the secretome of HEK293T cells. Most of the identified proteins were likely soluble or single‐pass transmembrane proteins, which may serve as specific proteins for the diagnosis using biological fluids. Our method may help to discover marker glycoproteins for various cancers generating WFA‐recognized glycans.