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Iron and FER‐LIKE IRON DEFICIENCY‐INDUCED TRANSCRIPTION FACTOR‐dependent regulation of proteins and genes in Arabidopsis thaliana roots
Author(s) -
Mai HansJörg,
Lindermayr Christian,
Toerne Christine,
FinkStraube Claudia,
Durner Jörg,
Bauer Petra
Publication year - 2015
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201400351
Subject(s) - arabidopsis thaliana , arabidopsis , transcription factor , mutant , iron deficiency , biology , iron homeostasis , gene , transcriptome , microbiology and biotechnology , regulation of gene expression , wild type , genetics , transcriptional regulation , gene expression , medicine , anemia
Iron is an essential micronutrient for plants, and iron deficiency requires a variety of physiological adaptations. FIT (FER‐LIKE IRON DEFICIENCY‐INDUCED TRANSCRIPTION FACTOR) is essential for the regulation of iron uptake in Arabidopsis thaliana roots. FIT is transcriptionally as well as posttranscriptionally regulated in response to iron supply. To investigate to which extent posttranscriptional regulation upon iron deficiency applies to proteins and to determine the dependency on FIT, we performed a parallel proteomic and transcriptomic study with wild‐type, a fit knock‐out mutant, and a FIT overexpressing Arabidopsis line. Among 92 proteins differentially regulated by iron and/or FIT, we identified 30 proteins, which displayed differential regulation at the transcriptional level. Eleven protein spots were regulated in at least one of the data points even contrary to the respective genes dependent on FIT. We found ten proteins in at least two forms. The analysis of functional classification showed enriched GO terms among the posttranscriptionally regulated genes and of proteins, that were downregulated or absent in the fit knock‐out mutant. Taken together, we provide evidence for iron and FIT‐dependent posttranscriptional regulation in iron homeostasis in A. thaliana .