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Transcriptome and proteome of human hepatocellular carcinoma reveal shared metastatic pathways with significant genes
Author(s) -
Shen Huali,
Zhong Fan,
Zhang Yang,
Yu Hongxiu,
Liu Yinkun,
Qin Lunxiu,
He Fuchu,
Tang Zhaoyou,
Yang Pengyuan
Publication year - 2015
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201400275
Subject(s) - transcriptome , gene , biology , proteome , wnt signaling pathway , hepatocellular carcinoma , metastasis , cancer research , gene expression , computational biology , gene expression profiling , genetics , cancer
Previously isolated pathways screened from individual genes were investigated at either the transcriptional or translational level; however, the consistency between the pathways screened at the gene expression levels was obscure in metastatic human hepatocellular carcinoma (HCC). To elucidate this question, we performed a transcriptomic (16 353 genes) and proteomic (7861 proteins) analysis simultaneously on six metastatic HCC cell lines against two nonmetastatic HCC cell lines, with all HBV traceable and close genetic‐backgrounds for a comparative study. The quantitative and integrated results showed that significant genes were screened differentially with 351 transcripts from the transcriptome and 304 proteins from the proteome, with limited overlapping genes (7%). However, we discovered that these discrete 351 transcripts and 304 proteins screened share extrusive significant‐pathways/networks with a 77% overlap, including active TGF‐β, RAS, NFκB, and Wnt, and inactive HNF4A, which are responsible for HCC metastasis. We conclude that the discrete, but significant genes predicted by either ome play intrinsically important roles in the linkage of responsible pathways shared by both omes in HCC metastasis.