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Array‐based proteomic approaches to study signal transduction pathways: Prospects, merits and challenges
Author(s) -
Gahoi Nikita,
Ray Sandipan,
Srivastava Sanjeeva
Publication year - 2015
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201400261
Subject(s) - interactome , protein microarray , computational biology , signal transduction , multiplex , protein–protein interaction , biology , dna microarray , proteomics , protein array analysis , bioinformatics , microbiology and biotechnology , genetics , gene expression , gene
Very often dysfunctional aspects of various signalling networks are found to be associated with human diseases and disorders. The major characteristics of signal transduction pathways are specificity, amplification of the signal, desensitisation and integration, which is accomplished not solely, but majorly by proteins. Array‐based profiling of protein–protein and other biomolecular interactions is a versatile approach, which holds immense potential for multiplex interactome mapping and provides an inclusive representation of the signal transduction pathways and networks. Protein microarrays such as analytical protein microarrays (antigen–antibody interactions, autoantibody screening), RP microarrays (interaction of a particular ligand with all the possible targets in cell), functional protein microarrays (protein–protein or protein–ligand interactions) are implemented for various applications, including analysis of protein interactions and their significance in signalling cascades. Additionally, successful amalgamation of the array‐based approaches with different label‐free detection techniques allows real‐time analysis of interaction kinetics of multiple interaction events simultaneously. This review discusses the prospects, merits and limitations of different variants of array‐based techniques and their promising applications for studying the modifications and interactions of biomolecules, and highlights the studies associated with signal transduction pathways and their impact on disease pathobiology.