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From pathways to networks: Connecting dots by establishing protein–protein interaction networks in signaling pathways using affinity purification and mass spectrometry
Author(s) -
Li Xu,
Wang Wenqi,
Chen Junjie
Publication year - 2015
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201400147
Subject(s) - computational biology , proteomics , protein–protein interaction , biological pathway , signal transduction , identification (biology) , systems biology , biology , microbiology and biotechnology , biochemistry , gene expression , botany , gene
Signal transductions are the basis of biological activities in all living organisms. Studying the signaling pathways, especially under physiological conditions, has become one of the most important facets of modern biological research. During the last decade, MS has been used extensively in biological research and is proven to be effective in addressing important biological questions. Here, we review the current progress in the understanding of signaling networks using MS approaches. We will focus on studies of protein–protein interactions that use affinity purification followed by MS approach. We discuss obstacles to affinity purification, data processing, functional validation, and identification of transient interactions and provide potential solutions for pathway‐specific proteomics analysis, which we hope one day will lead to a comprehensive understanding of signaling networks in humans.