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Plasma proteomic analysis of patients infected with H1N1 influenza virus
Author(s) -
Choi Seonyoung,
Lim Jiyoun,
Kim Yoonjin,
Song Moon Jung,
Jung WoonWon,
Seo Jung Bok,
Lee Jacob,
Sul Donggeun
Publication year - 2014
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201400030
Subject(s) - western blot , blood proteins , glycoprotein , virus , proteome , biology , downregulation and upregulation , influenza a virus , blot , proteomics , microbiology and biotechnology , virology , biochemistry , gene
This study profiled the plasma proteins of patients infected by the 2011 H1N1 influenza virus. Differential protein expression was identified in plasma obtained from noninfected control subjects ( n = 15) and H1N1‐infected subjects ( n = 15). Plasma proteins were separated by a 2DE large gel system and identified by nano‐ultra performance LC‐MS. Western blot assays were performed to validate proteins. Eight plasma proteins were upregulated and six proteins were downregulated among 3316 plasma proteins in the H1N1‐infected group as compared with the control group. Of 14 up‐ and downregulated proteins, nine plasma proteins were validated by Western blot analysis. Putative protein FAM 157A, leucine‐rich alpha 2 glycoprotein, serum amyloid A protein, and dual oxidase 1 showed significant differential expression. The identified plasma proteins could be potential candidates for biomarkers of H1N1 influenza viral infection. Further studies are needed to develop these proteins as diagnostic biomarkers.