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Proteomic analyses of genes regulated by heterogeneous nuclear ribonucleoproteins A / B in J urkat cells
Author(s) -
Yeh YuanMing,
Chen ChiYuan,
Huang PeiRong,
Hsu ChiaWei,
Wu ChihChing,
Wang TzuChien V.
Publication year - 2014
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201300549
Subject(s) - heterogeneous nuclear ribonucleoprotein , heterogeneous ribonucleoprotein particle , microbiology and biotechnology , ribonucleoprotein , proteome , biology , cell growth , cytoskeleton , rhoa , rna binding protein , chemistry , cell , rna , gene , signal transduction , genetics
Several lines of evidence suggest that hnRNPs A/B (hnRNPs A1, A 2/ B 1, and A 3) play an important role in proliferation, although the functional overlap among members of hn RNPs A / B remains largely unknown. In this study, we have employed RNA i knockdown and proteomic approaches to investigate the biological functions of hn RNPs A / B . Depletion of hnRNP A2, but not A1 or A3, produced a significant inhibition of cellular proliferation in J urkat cells. Analysis of the proteomes in the cells depleted for hnRNP A1, A2, or A3 has identified a total of 167 differentially expressed proteins in the depleted cells. Network analysis of the proteins altered in the cells depleted for hnRNP A2 revealed that the biological processes likely affected by these proteins are related to cell cycle, cytoskeleton rearrangement, and transcription regulation. Indeed, we have confirmed that the level of RhoA and CrkL was selectively reduced in the cells depleted of hnRNP A2, but not in the cells depleted for hnRNP A1 or A3. Therefore, we suggest that the reduced proliferation observed in the cells depleted of hnRNP A2 may result from its effects on cell adhesion processes in the J urkat cells.