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Proteomic characterization of seeds from yellow lupin ( L upinus luteus L .)
Author(s) -
Ogura Takahiro,
Ogihara Jun,
Sunairi Michio,
Takeishi Hidetaka,
Aizawa Tomoko,
OlivosTrujillo Marcos R.,
MaureiraButler Iván J.,
SalvoGarrido Haroldo E.
Publication year - 2014
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201300511
Subject(s) - lupinus , storage protein , biology , legume , spots , proteomics , kegg , proteome , botany , biochemistry , gene , gene ontology , gene expression
Yellow lupin ( L upinus luteus L .) is a legume crop containing a large amount of protein in its seeds. In this study, we constructed a seed‐protein catalog to provide a foundation for further study of the seeds. A total of 736 proteins were identified in 341 2 DE spots by nano‐ LC ‐ MS / MS . Eight storage proteins were found as multiple spots in the 2 DE gels. The 736 proteins correspond to 152 unique proteins as shown by U ni R ef50 clustering. Sixty‐seven of the 152 proteins were associated with KEGG ‐defined pathways. Of the remaining proteins, 57 were classified according to a GO term. The functions of the remaining 28 proteins have yet to be determined. This is the first yellow lupin seed–protein catalog, and it contains considerably more data than previously reported for white lupin ( L . albus L .).