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In macropore tryptic digestion at acidic p H and its implication for proteomics
Author(s) -
Medvedev Alexei E.
Publication year - 2013
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201300440
Subject(s) - myoglobin , digestion (alchemy) , proteomics , trypsin , chemistry , shotgun proteomics , chromatography , biochemistry , enzyme , gene
Gan et al. (Proteomics 2013, 13, 3117–3123) described a new “macropore” protocol for effective protein digestion by trypsin suitable for a wide range of pH including acidic pH. It was effective not only in experiments with solutions of a model protein (myoglobin), but also with a subfraction of rat liver cytosol. This significantly simplifies and accelerates protein digestion procedures for subsequent MS . However, further studies are needed to find limits of experimental applicability of the described protocol in proteomics.