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Cellular response to empty and palladium‐conjugated amino‐polystyrene nanospheres uptake: A proteomic study
Author(s) -
Pietrovito Laura,
CanoCortés Victoria,
Gamberi Tania,
Magherini Francesca,
Bianchi Laura,
Bini Luca,
SánchezMartín Rosario M.,
Fasano Mauro,
Modesti Alessandra
Publication year - 2015
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201300423
Subject(s) - hek 293 cells , intracellular , conjugated system , cell , chemistry , cell culture , prodrug , regulator , microbiology and biotechnology , biochemistry , biology , gene , genetics , organic chemistry , polymer
Amino polystyrene nanospheres are shown to be efficient and controllable delivery devices, capable of transporting several bioactive cargoes. Recently, the design of a new device for prodrug activation, using these nanospheres with palladium encapsulated onto them, has been developed successfully. To study the influence of the cellular uptake of these nanodevices, we investigated the cellular response of human embryonic kidney cells (HEK‐293T) and murine fibroblasts (L929) treated with empty or palladium‐conjugated amino polystyrene nanospheres. To identify differentially expressed proteins, we performed an exhaustive proteomic analysis. In accordance with genomic data previously obtained, the uptake of the empty nanospheres did not induce significant variation in protein expression levels. Following the treatment with palladium‐conjugated nanospheres, some changes in protein profiles in both cell lines were observed; these alterations affect proteins involved in cell metabolism and intracellular transport. No key regulator of the cell cycle result was differentially expressed after the treatment, confirming that these innovative drug delivery systems are harmless and well tolerated by the cells.